Ablation of a subpopulation of neurons using a two-photon laser

A pretectal nucleus (nucleus pretectalis superficialis pars magnocellularis, or PSm) was laser ablated bilaterally using Zeiss two photon laser microscope. The movie shows EGFP fluorescent images of a 4 day old zebrafish larval brain before (left) and after (right) laser ablation. Forebrain, tectum, pretectum (PSm), preglomerular nuclei, and cerebellum are genetically labeled in this gal4 line, gSAIzGFFM119B.



  1. Muto et al., 2017. Activation of the hypothalamic feeding centre upon visual prey detection https://www.nature.com/articles/ncomms15029