Behavioral recording of zebrafish larvae in a multiwell plate

Behavioral recording

When we record from many zebrafish larvae in multi-wells, the wall of the wells may hide the larvae. This problem can be solved by the use of a telecentmeric lens that has a huge size to cover the entire area where the wells are present. Here is an example of a recording from 72 zebrafish larvae in a 96 well plate.

Unfortunately, the lens used here does not cover the entire plate. The 25MP camera has a enough resolution to see the eyes of the individual larva. Below is a single well zoomed in.

Lens: . TC16M120 Telecentric lens for 35 mm detectors, magnification 0.289 x, F-mount

Camera: CL25000CXP

Camera board: Active Silicon AS-FBD-4XCXP6-2PE8

Things to be considered

  1. One frame from a 25MP camera has a file size of 25MB.  It may not be realistic to save a movie file from the time-lapse recording and analyze it later. Real-time image processing may be preferred.
  2. The image shown above was taken with a sample application that came with the camera. There seems no camera acquisition software for this coaxpress camera.
  3. Active Silicon NOW (, but not in 2013,) supports National Instruments LabVIEW ( Writing a custom script (VI, Virtual Instrument) in LabVIEW may be the way to go.