Behavioral recording of zebrafish larvae in a multiwell plate

Behavioral recording

When we record from many zebrafish larvae in multi-wells, the wall of the wells may hide the larvae. This problem can be solved by the use of a telecentmeric lens that has a huge size to cover the entire area where the wells are present. Here is an example of a recording from 72 zebrafish larvae in a 96 well plate.

Unfortunately, the lens used here does not cover the entire plate. The 25MP camera has a enough resolution to see the eyes of the individual larva. Below is a single well zoomed in.

Lens: . TC16M120 Telecentric lens for 35 mm detectors, magnification 0.289 x, F-mount http://www.opto-engineering.com/products/very-large-detector-tc-lens-model-TC16M120

Camera: CL25000CXP https://www.stemmer-imaging.co.uk/en/news/2012-06-optronis-cl25000cxp-53-images-per-second-with-25-megapixels/

Camera board: Active Silicon AS-FBD-4XCXP6-2PE8 https://www.activesilicon.com/products/firebird-coaxpress-frame-grabber-quad-CXP-6/

Things to be considered

  1. One frame from a 25MP camera has a file size of 25MB.  It may not be realistic to save a movie file from the time-lapse recording and analyze it later. Real-time image processing may be preferred.
  2. The image shown above was taken with a sample application that came with the camera. There seems no camera acquisition software for this coaxpress camera.
  3. Active Silicon NOW (, but not in 2013,) supports National Instruments LabVIEW (https://www.activesilicon.com/products/labview-driver/). Writing a custom script (VI, Virtual Instrument) in LabVIEW may be the way to go.

 

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