NIG course for Zebrafish Imaging and Transgenesis 2015

NIG course for Zebrafish Imaging and Transgenesis was held at National Institute of Genetics (NIG) in Mishima, Japan during July 29-Aug 8, 2015. It was sponsored by NIG, and hosted by Koichi Kawakami’s lab. The first half of the course was devoted to CRISPR/Cas9-mediated DNA editing for mutagenesis and knock-in. In the second half, we performed calcium imaging of the zebrafish larval brain.

For the training course, we brought all the stuff needed into an empty room: lab equipment, five stereomicroscopes, and five epifluorescence microscopes equipped with Hamamatsu cameras.

20150806_LabSetupPic1

Microscope:ZEISS Examiner D.1, Objective lens: IR-ACHROPLAN 40x/0,80W 440095, Camera:HAMAMATSU ORCA-Flash4.0, Desktop PC:DELL Precision T5500, Laptop PC:FRONTIER, DAQ:NI USB-6001
Microscope1_ZeissExaminer

Microscope:NIKON, Camera:Hamamatsu ORCA-R2, Desktop PC:DELL Precision 5500, Laptop PC:FRONTIER, DAQ:NI USB-6001
Microscope2_Nikon

Microscope:OLYMPUS BX53, Camera:Hamamatsu ORCA-R2,  Desktop PC:DELL Precision 5500, Laptop PC: NEC LaVie (Windows8), DAQ:NI USB-6001
Microscope3_Olympus

Microscope: OLYMPUS BX51WI, Camera: HAMAMATSU ORCA-Flash4.0, Desktop PC:DELL Precision 5500, Laptop PC: IIYAMA, DAQ:NI USB-6001
Microscope4_Olympus

Microscope: ZEISS Imager Z.2, Camera: HAMAMATSU ORCA-R2, Desktop PC:DELL Precision T5500, Laptop PC:FRONTIER, DAQ:NI USB-6001
Microscope5_ZeissImager

Image acquisition software: HCImage with High Speed Streaming module (ORCA-R2) or Hard Disk Record module (ORCA-Flash4.0) (Hamamatsu Photonics)
Software for visual stimulus presentation: Matlab R2015a with Psychtoolbox-3

We are very grateful to Hamamatsu Photonics, Zeiss, Olympus and Nikon for lending us the microscopes and cameras for this training course.

Web resources

  1. NIG course for Zebrafish Imaging and Transgenesis (nig.ac.jp)
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