Yokogawa CSU-W1 confocal laser microscope Z-stack acquisition in the Yokogawa CSU-W1 confocal microscope
The image acquisition software Andor iQ3 employed in the Yokogawa SU-W1 confocal microscope dose not seem very user-frie...
Yokogawa CSU-W1 confocal laser microscope 
Behavioral recording Custom-made optokinetic drum for the recording of the optokinetic response (OKR) in zebrafish larvae
At the time when I was at UCSF in Baier lab, the lab was using a projector to make the optokinetic stimuli (Roeser and B...
Calcium imaging Zebrafish calcium imaging using a Zeiss LightSheet microsope
Our institute, National Institute of Genetics, has just bought a Zeiss lightsheet microscope. So, I wanted to see how it...
未分類 Controlling LED light and electrical shock via a transistor-relay circuit
In the two-way active avoidance experiment, LEDs were used as a conditioned stimulus (CS), and the electrical shock was ...
Python scripts How to automate averaging paramecium counts in each csv file in a folder using python
Previously, I took short movie of parameia and counted them in Fiji/ImageJ and now I have a lot of csv files (Summary of...
ImageJ Macors Counting paramecia in Fiji/ImageJ with pieces of macros
ImageJ Macro writing takes some time. When the number of the files to be processed is not extremely large, doing it manu...
Laser ablation Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in Zebrafish Larvae
Abstract
To identify the role of a subpopulation of neurons in behavior, it is essential to test the consequences o...
Publication Identification of a neuronal population in the telencephalon essential for fear conditioning in zebrafish
Finally, our Pradeep's paper was published, in BMC Biology, after a long, long journey.
Press releases
How c...
Protocols A protocol for DNA injection to generate transgenic zebrafish
We often make transgenic zebrafish lines by injecting a DNA construct that uses a Tol2 transposon vector, into eggs and ...
Paramecium How to culture paramecia: a very simple method using KIRIN Namacha（キリン生茶）
We previously described a method of paramecium culture that we use in our lab: "Calcium Imaging of Neuronal Activity in ...
Behavioral recording Behavioral recording of zebrafish larvae in a multiwell plate
When we record from many zebrafish larvae in multi-wells, the wall of the wells may hide the larvae. This problem can be...
Laser ablation Ablation of a subpopulation of neurons using a two-photon laser
A pretectal nucleus (nucleus pretectalis superficialis pars magnocellularis, or PSm) was laser ablated bilaterally using...
Image registration Navigation in zebrafish larvae
Zebrafish larva and paramecia
File:20140912 165201
Recording: Navigation in a small chamber (20 mm diameter) w...
Publication “Activation of the hypothalamic feeding centre upon visual prey detection” published in Nature Communications
Our latest paper on prey capture, "Activation of the hypothalamic feeding centre upon visual prey detection" was publis...
Lab pictures Zebrafish pictures
All the following pictures can be used under CC BY 4.0. Credit should be given to the National Institute of Genetics (©...
prey capture Activation of the hypothalamic feeding centre upon visual prey detection
I uploaded a submitted manuscript onto the BioRxiv and some of the supplementary movies on Youtube.
Akira Muto, Prade...
Batch processing How to read Hamamatsu Photonics CXD files in ImageJ and save them as TIFF files automatically
I am using Hamamatsu Photonics ORCA Flash 4.0 with the accompanied image acquisition software, HCImage. After time-lapse...
National Instruments LabVIEW NI-IMAQdx missing in the function palette
I thought I installed everything needed for camera control but I found that NI-IMAQdx subVIs were missing in the functio...
ImageJ Macors Prey capture assay for zebrafish larvae ~ Counting the number of the paramecia
To quantitatively describe the ability of prey capture in zebrafish larvae, I need to count the number of paramecia cons...
ImageJ Prey capture assay for zebrafish larvae ~ The lighting system
To understand how a sensory input leads to a particular behavioral output, we are studying prey capture behavior in zebr...