Behavioral recording Behavioral recording of zebrafish larvae in a multiwell plate
When we record from many zebrafish larvae in multi-wells, the wall of the wells may hide the larvae. This problem can be...
Behavioral recording 
Laser ablation Ablation of a subpopulation of neurons using a two-photon laser
A pretectal nucleus (nucleus pretectalis superficialis pars magnocellularis, or PSm) was laser ablated bilaterally using...
Image registration Navigation in zebrafish larvae
Zebrafish larva and paramecia
File:20140912 165201
Recording: Navigation in a small chamber (20 mm diameter) w...
Publication “Activation of the hypothalamic feeding centre upon visual prey detection” published in Nature Communications
Our latest paper on prey capture, "Activation of the hypothalamic feeding centre upon visual prey detection" was publis...
Lab pictures Zebrafish pictures
All the following pictures can be used under CC BY 4.0. Credit should be given to the National Institute of Genetics (©...
prey capture Activation of the hypothalamic feeding centre upon visual prey detection
I uploaded a submitted manuscript onto the BioRxiv and some of the supplementary movies on Youtube.
Akira Muto, Prade...
Batch processing How to read Hamamatsu Photonics CXD files in ImageJ and save them as TIFF files automatically
I am using Hamamatsu Photonics ORCA Flash 4.0 with the accompanied image acquisition software, HCImage. After time-lapse...
National Instruments LabVIEW NI-IMAQdx missing in the function palette
I thought I installed everything needed for camera control but I found that NI-IMAQdx subVIs were missing in the functio...
ImageJ Macors Prey capture assay for zebrafish larvae ~ Counting the number of the paramecia
To quantitatively describe the ability of prey capture in zebrafish larvae, I need to count the number of paramecia cons...
ImageJ Prey capture assay for zebrafish larvae ~ The lighting system
To understand how a sensory input leads to a particular behavioral output, we are studying prey capture behavior in zebr...
Batch processing Batch processing of behavioral data ~ How to anlyze locations of individual animals
In behavioral studies, we want to analyze the locomotion. If we work on a single individual, we can easily obtain the po...
Publication Motionless prey is invisible to predators
We visualized neuronal activity in the optic tectum of a zebrafish larva during prey perception. A moving paramecium evo...
Zebrafish ethogram zebrafish larvae as a model system for the study of cognitive functions
Zebrafish larvae capture a small object. This is a very interesting behavior because it consists of several neural proce...
ImageJ How to make a background image in the presence of the larva
Sometimes I need to put zebrafish larva in the recording chamber from the beginning of a recording and still want a back...
Image registration Image registration with TurboReg in ImageJ
During the time-lapse recording, the zebrafish larvae often make movements even in the agarose. Therefore, we need to r...
ImageJ How to make a graph of the calcium signals from multiple neurons using ImageJ (Fiji) and Excel
Here is what I would do to plot calcium signals from multiple neurons after time-lapse imaging. The brain images (GCaMP ...
未分類 NIG course for Zebrafish Imaging and Transgenesis 2015
NIG course for Zebrafish Imaging and Transgenesis was held at National Institute of Genetics (NIG) in Mishima, Japan dur...
Visual stimulus presentation A movie theater for zebrafish larvae under the microscope
There could be several ways to give visual stimuli to zebrafish. Using a LCD display, an OLED display, a projector, and ...
Visual stimulus presentation Making of a recording chamber: A TV theater for zebrafish larvae
At DAISO (a 100-yen shop / one-dollar shop), I found a small collection box (approx. 6 cm x 6 cm x 5 cm height) that ca...
Objective lenses 40x Water dipping objective lenses ~ How deep can you go?
I had an opportunity to use water dipping objective lenses from different manufacturers and was surprised to learn that ...