Prey capture assay for zebrafish larvae ~ The lighting system

To understand how a sensory input leads to a particular behavioral output, we are studying prey capture behavior in zebr...

How to make a background image in the presence of the larva

Sometimes I need to put zebrafish larva in the recording chamber from the beginning of a recording and still want a back...
Image registration

Image registration with TurboReg in ImageJ

During the time-lapse recording, the zebrafish larvae often make movements even  in the agarose. Therefore, we need to r...

How to make a graph of the calcium signals from multiple neurons using ImageJ (Fiji) and Excel

Here is what I would do to plot calcium signals from multiple neurons after time-lapse imaging. The brain images (GCaMP ...