akira

Lab pictures

Zebrafish pictures

All the following pictures can be used under CC BY 4.0.  Credit should be given to the National Institute of Genetics (©...
prey capture

Activation of the hypothalamic feeding centre upon visual prey detection

I uploaded a submitted manuscript onto the BioRxiv and some of the supplementary movies on Youtube. Akira Muto, Prade...
Batch processing

How to read Hamamatsu Photonics CXD files in ImageJ and save them as TIFF files automatically

I am using Hamamatsu Photonics ORCA Flash 4.0 with the accompanied image acquisition software, HCImage. After time-lapse...
National Instruments LabVIEW

NI-IMAQdx missing in the function palette

I thought I installed everything needed for camera control but I found that NI-IMAQdx subVIs were missing in the functio...
ImageJ Macors

Prey capture assay for zebrafish larvae ~ Counting the number of the paramecia

To quantitatively describe the ability of prey capture in zebrafish larvae, I need to count the number of paramecia cons...
ImageJ

Prey capture assay for zebrafish larvae ~ The lighting system

To understand how a sensory input leads to a particular behavioral output, we are studying prey capture behavior in zebr...
Batch processing

Batch processing of behavioral data ~ How to anlyze locations of individual animals

In behavioral studies, we want to analyze the locomotion. If we work on a single individual, we can easily obtain the po...
Publication

Motionless prey is invisible to predators

We visualized neuronal activity in the optic tectum of a zebrafish larva during prey perception. A moving paramecium evo...
Zebrafish ethogram

zebrafish larvae as a model system for the study of cognitive functions

Zebrafish larvae capture a small object. This is a very interesting behavior because it consists of several neural proce...
ImageJ

How to make a background image in the presence of the larva

Sometimes I need to put zebrafish larva in the recording chamber from the beginning of a recording and still want a back...
Image registration

Image registration with TurboReg in ImageJ

During the time-lapse recording, the zebrafish larvae often make movements even  in the agarose. Therefore, we need to r...
ImageJ

How to make a graph of the calcium signals from multiple neurons using ImageJ (Fiji) and Excel

Here is what I would do to plot calcium signals from multiple neurons after time-lapse imaging. The brain images (GCaMP ...
未分類

NIG course for Zebrafish Imaging and Transgenesis 2015

NIG course for Zebrafish Imaging and Transgenesis was held at National Institute of Genetics (NIG) in Mishima, Japan dur...
Visual stimulus presentation

A movie theater for zebrafish larvae under the microscope

There could be several ways to give visual stimuli to zebrafish. Using a LCD display, an OLED display, a projector, and ...
Visual stimulus presentation

Making of a recording chamber: A TV theater for zebrafish larvae

At DAISO (a 100-yen shop / one-dollar shop), I found a small collection box (approx. 6 cm x 6 cm x 5 cm height)  that ca...
Objective lenses

40x Water dipping objective lenses ~ How deep can you go?

I had an opportunity to use water dipping objective lenses from different manufacturers and was surprised to learn that ...
Publication

Real-Time Visualization of Neuronal Activity during Perception (Current Biology 2013)

Neuronal Activity during Visual Perception 脳に映る視覚世界をリアルタイムで観察 Tectal responses during perception of a swimming para...
Bubble capture behavior

Bubble capture behavioral assay

I recorded a single larva for a long time (three hours) for the first time to see how behavior changes over time. The be...
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