akira

科研費

採択される科研費申請書の書き方に関する考察

第17回東邦大学5学部合同学術風会抄録集 第17回 東邦大学5学部合同学術集会 口頭発表 オンライン開催 2021年3月13日(土) 13:00~15:50 採択される科研費申請書の書き方に関する考察 -2年間の添削経験をもとに-...
Lab pictures

Saving frames when the animal (fish) is in the camera view

Zebrafish larvae swim intermittently, with a pause between bouts. Calcium signals can be obtained when fish are not movi...
未分類

Fluorescent Callibration Slides

Product memo: Applied Precision , Inc. Fluorescent Calibration Slide Ex.408, Em. 440 Fluorescent Calibration Sl...
Behavioral recording

Papers on behavior of larval zebrafish

Phototaxis Zebrafish larvae show negative phototaxis to near-infrared light. Hartmann S, Vogt R, Kunze J, Rauschert A...
Yokogawa CSU-W1 confocal laser microscope

Z-stack acquisition in the Yokogawa CSU-W1 confocal microscope

The image acquisition software Andor iQ3 employed in the Yokogawa SU-W1 confocal microscope dose not seem very user-frie...
Behavioral recording

Custom-made optokinetic drum for the recording of the optokinetic response (OKR) in zebrafish larvae

At the time when I was at UCSF in Baier lab, the lab was using a projector to make the optokinetic stimuli (Roeser and B...
Calcium imaging

Zebrafish calcium imaging using a Zeiss LightSheet microsope

Our institute, National Institute of Genetics, has just bought a Zeiss lightsheet microscope. So, I wanted to see how it...
未分類

Controlling LED light and electrical shock via a transistor-relay circuit

In the two-way active avoidance experiment, LEDs were used as a conditioned stimulus (CS), and the electrical shock was ...
Python scripts

How to automate averaging paramecium counts in each csv file in a folder using python

Previously, I took short movie of parameia and counted them in Fiji/ImageJ and now I have a lot of csv files (Summary of...
ImageJ Macors

Counting paramecia in Fiji/ImageJ with pieces of macros

ImageJ Macro writing takes some time. When the number of the files to be processed is not extremely large, doing it manu...
Laser ablation

Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in Zebrafish Larvae

Abstract To identify the role of a subpopulation of neurons in behavior, it is essential to test the consequences o...
Publication

Identification of a neuronal population in the telencephalon essential for fear conditioning in zebrafish

Finally, our Pradeep's paper was published, in BMC Biology, after a long, long journey. Press releases How c...
Protocols

A protocol for DNA injection to generate transgenic zebrafish

We often make transgenic zebrafish lines by injecting a DNA construct that uses a Tol2 transposon vector, into eggs and ...
Paramecium

How to culture paramecia: a very simple method using KIRIN Namacha(キリン生茶)

We previously described a method of paramecium culture that we use in our lab: "Calcium Imaging of Neuronal Activity in ...
Behavioral recording

Behavioral recording of zebrafish larvae in a multiwell plate

When we record from many zebrafish larvae in multi-wells, the wall of the wells may hide the larvae. This problem can be...
Laser ablation

Ablation of a subpopulation of neurons using a two-photon laser

A pretectal nucleus (nucleus pretectalis superficialis pars magnocellularis, or PSm) was laser ablated bilaterally using...
Image registration

Navigation in zebrafish larvae

Zebrafish larva and paramecia File:20140912 165201 Recording: Navigation in a small chamber (20 mm diameter) w...
Publication

“Activation of the hypothalamic feeding centre upon visual prey detection” published in Nature Communications

Our latest paper on prey capture,  "Activation of the hypothalamic feeding centre upon visual prey detection" was publis...
Lab pictures

Zebrafish pictures

All the following pictures can be used under CC BY 4.0.  Credit should be given to the National Institute of Genetics (©...
prey capture

Activation of the hypothalamic feeding centre upon visual prey detection

I uploaded a submitted manuscript onto the BioRxiv and some of the supplementary movies on Youtube. Akira Muto, Prade...
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